The pE-300ultra is the most controllable member of the pE-300 Series for LED microscope lighting.  In addition to the many features of the pE-300lite and the pE-300white, the system offers precise control over wavelength irradiance and shuttering.  Until now these benefits have only been accessible to users of high end, highly flexible LED microscope illumination systems such as the CoolLED pE-4000.

It allows adjustment of output in 1% steps, giving precise control.  The broad spectrum covers everyday fluorophores: DAPI, CFP, Aqua, FITC, TRITC, TxRed, Cy5 and many more.

Direct fit or liquid light guide

There are two pE-300ultra configuration options: direct fit or liquid light guide. The Direct Fit variant delivers higher irradiance and reduces the cost to buy and run thanks to fewer consumables.

For confocal microscopes, the Liquid Light Guide model offers the ideal replacement to a metal halide light source, minimising photobleaching and phototoxicity when viewing samples before scanning. More information on the available variants can be found under specifications.

Capturing high-speed events

The pE-300ultra offers triggering from multiple TTL inputs which, coupled with the ability to mount inline excitation filters, provides microsecond switching of pre-filtered excitation light. When paired with today’s high performance multi-band filter sets, this facilitates imaging traditionally achieved using a white light source and a filter wheel, with all the benefits of LEDs – and most excitingly at speeds not previously so affordable. For live cell imaging applications, events can now be captured that might previously have been missed. Read our white paper to compare the benefits and trade-offs of different possible illumination configurations.

Fast multi-channel imaging

Fluorophores can be viewed either individually or in combination, without filter cube changes.  This makes the pE-300ultra ideal for use with multi-band filter sets as the screening process can be simplified when fewer filter cubes are used.  Independent control of the three LED channels means that the user can control the level of excitation of each fluorophore independently on a multi-stained sample, potentially removing the need for single-band filter sets altogether.

Sequence runner for multi-channel excitation

It is now possible to run through a sequence of excitation channels using a camera or other external hardware which has only a single TTL-out, thanks to the Sequence Runner mode. Complex illumination protocols can now be run at high speeds and without the need specialised and costly hardware. The order of each fluorophore to be captured is defined via the simple Control Pod, with the pE-300ultra Light Source accepting a single TTL output from the experiment set-up’s camera to initiate the step-through of a sequence of excitation channels.  This feature is independent of the individual channel TTL inputs on the Light Source. Find out more about this useful feature in our Sequence Runner tutorial video.

Energy efficiency for cost-saving sustainability

When it comes to making labs safer and more sustainable, LED microscope lighting is an attractive option when compared to old toxic mercury or metal halide lamps. All three channels of the pE-300ultra at full power use just 60 Watts, which is even lower than other LED technology which uses 120 to 350 Watts. “Green Grants” are even sometimes available from establishments, providing financial support owing to the reduction in institutional energy usage.